3D_analysis.ipynb

{
  "cells": [
    {
      "cell_type": "code",
      "execution_count": 1,
      "metadata": {
        "id": "ES1OBpKdEASK"
      },
      "outputs": [],
      "source": [
        "%%capture\n",
        "!pip install SimpleITK\n",
        "# !pip install skan\n",
        "!pip install git+https://github.com/jni/skan\n",
        "!pip install tifffile"
      ]
    },
    {
      "cell_type": "code",
      "execution_count": 6,
      "metadata": {
        "id": "IrZnR3HhEEWy"
      },
      "outputs": [],
      "source": [
        "import SimpleITK as sitk\n",
        "from skimage.morphology import skeletonize, thin, medial_axis#, skeletonize_3d\n",
        "from scipy import ndimage\n",
        "from skan import csr, Skeleton\n",
        "from skan import summarize\n",
        "from skan import draw\n",
        "import tifffile as tiff\n",
        "import asyncio\n",
        "\n",
        "import matplotlib.pyplot as plt\n",
        "from mpl_toolkits.mplot3d import Axes3D\n",
        "import numpy as np\n",
        "import cv2\n",
        "import plotly.express as px\n",
        "import plotly.graph_objects as go\n",
        "import pandas as pd\n",
        "import glob\n",
        "from tqdm.notebook import tnrange\n",
        "import shutil\n",
        "from google.colab import files\n",
        "import os"
      ]
    },
    {
      "cell_type": "code",
      "source": [
        "def plot_3d_nodes_and_branches_mpl(df_nodes, df_segments):\n",
        "    \"\"\"Plots the 3D nodes and branches using Matplotlib.\"\"\"\n",
        "    fig = plt.figure(figsize=(10, 10))\n",
        "    ax = fig.add_subplot(111, projection='3d')\n",
        "\n",
        "    # Plot nodes\n",
        "    ax.scatter(df_nodes['node_coordinate_x'], df_nodes['node_coordinate_y'], df_nodes['node_coordinate_z'], c='red', marker='o', label='Nodes')\n",
        "\n",
        "    # Plot branches\n",
        "    for _, row in df_segments.iterrows():\n",
        "        path = np.array(row['path-coords'])\n",
        "        ax.plot(path[:, 2], path[:, 1], path[:, 0], color='blue', linewidth=1)\n",
        "\n",
        "    ax.set_xlabel('X')\n",
        "    ax.set_ylabel('Y')\n",
        "    ax.set_zlabel('Z')\n",
        "    plt.legend()\n",
        "    plt.show()\n",
        "\n",
        "\n",
        "\n",
        "def plot_3d_nodes_and_branches_plotly(df_nodes, df_segments):\n",
        "    \"\"\"\n",
        "    Plots 3D nodes and branches using Plotly.\n",
        "\n",
        "    Args:\n",
        "        df_nodes (pd.DataFrame): DataFrame containing node data.\n",
        "        df_segments (pd.DataFrame): DataFrame containing segments data.\n",
        "    \"\"\"\n",
        "    fig = go.Figure()\n",
        "\n",
        "    # Plot nodes\n",
        "    fig.add_trace(go.Scatter3d(\n",
        "        x=df_nodes['node_coordinate_x'],\n",
        "        y=df_nodes['node_coordinate_y'],\n",
        "        z=df_nodes['node_coordinate_z'],\n",
        "        mode='markers',\n",
        "        marker=dict(size=5, color='red'),\n",
        "        name='Nodes'\n",
        "    ))\n",
        "\n",
        "    # Plot branches\n",
        "    for _, row in df_segments.iterrows():\n",
        "        path = np.array(row['path-coords'])\n",
        "        if path.shape[1] == 3:  # Ensure correct shape\n",
        "            fig.add_trace(go.Scatter3d(\n",
        "                x=path[:, 2], y=path[:, 1], z=path[:, 0],  # Swap coordinates to match nodes\n",
        "                mode='lines',\n",
        "                line=dict(color='blue', width=2),\n",
        "                name='Branches'\n",
        "            ))\n",
        "\n",
        "    fig.update_layout(\n",
        "        scene=dict(\n",
        "            xaxis_title='X',\n",
        "            yaxis_title='Y',\n",
        "            zaxis_title='Z'\n",
        "        ),\n",
        "        title='3D Nodes and Branches Visualization'\n",
        "    )\n",
        "    fig.show()\n",
        "\n",
        "\n",
        "def tiffs_to_3d_numpy(folderpath):\n",
        "    \"\"\"\n",
        "    Converts tiff files to a 3D numpy array.\n",
        "\n",
        "    Args:\n",
        "        folderpath (str): path to folder containing tiff files.\n",
        "\n",
        "    Returns:\n",
        "        np.array: 3D numpy array containing image data.\n",
        "    \"\"\"\n",
        "    filelist = [os.path.join(folderpath, f) for f in os.listdir(folderpath) if os.path.isfile(os.path.join(folderpath, f))]\n",
        "\n",
        "    # Sort files by numerical order based on filename\n",
        "    filelist.sort(key=lambda x: int(x[-8:-5]))  # Sort by the 3-digit number in filenames\n",
        "\n",
        "    sorted_filelist = [f for f in filelist]\n",
        "\n",
        "    # Read the TIFF images into a numpy array\n",
        "    return np.array([plt.imread(fname) for fname in sorted_filelist])\n",
        "\n",
        "\n",
        "def compute_porosity(img):\n",
        "    \"\"\"\n",
        "    Computes porosity as the ratio of black pixels to total pixels.\n",
        "\n",
        "    Args:\n",
        "        img (np.array): binary image (0 for black, 1 for white).\n",
        "\n",
        "    Returns:\n",
        "        float: Porosity value.\n",
        "    \"\"\"\n",
        "    num_white_pix = np.sum(img == 1)\n",
        "    num_black_pix = np.sum(img == 0)\n",
        "    return num_black_pix / (num_white_pix + num_black_pix)\n",
        "\n",
        "\n",
        "def compute_distance_transform(segmentation_data):\n",
        "    \"\"\"\n",
        "    Computes the 3D distance transform of the segmentation data.\n",
        "\n",
        "    Args:\n",
        "        segmentation_data (np.array): 3D binary segmentation data.\n",
        "\n",
        "    Returns:\n",
        "        np.array: 3D distance transform of the binary image.\n",
        "    \"\"\"\n",
        "    return ndimage.distance_transform_edt(segmentation_data)\n",
        "\n",
        "\n",
        "def skeletonize_data(segmentation_data, dist_trans_3d):\n",
        "    \"\"\"\n",
        "    Skeletonizes the binary image and summarizes the skeleton using Skan.\n",
        "\n",
        "    Args:\n",
        "        segmentation_data (np.array): Binary image data.\n",
        "        dist_trans_3d (np.array): 3D distance transform of the binary image.\n",
        "\n",
        "    Returns:\n",
        "        pd.DataFrame: DataFrame containing skeleton data.\n",
        "    \"\"\"\n",
        "    skimage_skeleton = skeletonize(segmentation_data, method='lee')\n",
        "    skan_skeleton = Skeleton(skeleton_image=skimage_skeleton * dist_trans_3d)\n",
        "    df_skeleton = summarize(skan_skeleton)\n",
        "\n",
        "    # Rename and scale columns\n",
        "    df_skeleton.rename(columns={\n",
        "        'image-coord-src-0': 'src-x',\n",
        "        'image-coord-src-1': 'src-y',\n",
        "        'image-coord-src-2': 'src-z',\n",
        "        'image-coord-dst-0': 'dst-x',\n",
        "        'image-coord-dst-1': 'dst-y',\n",
        "        'image-coord-dst-2': 'dst-z',\n",
        "        'mean-pixel-value': 'thickness'\n",
        "    }, inplace=True)\n",
        "\n",
        "    # Scale thickness and distances\n",
        "    df_skeleton[['branch-distance', 'euclidean-distance']] *= (1.485)\n",
        "    df_skeleton[['thickness']] *= (2 * 1.485)\n",
        "\n",
        "    # Drop unnecessary columns\n",
        "    df_skeleton.drop(columns=['stdev-pixel-value', 'coord-src-0', 'coord-src-1', 'coord-src-2', 'coord-dst-0', 'coord-dst-2', 'coord-dst-1'], inplace=True)\n",
        "\n",
        "    return df_skeleton\n",
        "\n",
        "\n",
        "def compute_branch_coordinates(df_skeleton, skan_skeleton):\n",
        "    \"\"\"\n",
        "    Computes the 3D path coordinates for branches in the skeleton.\n",
        "\n",
        "    Args:\n",
        "        df_skeleton (pd.DataFrame): DataFrame containing skeleton data.\n",
        "        skan_skeleton (Skeleton): Skan skeleton object.\n",
        "\n",
        "    Returns:\n",
        "        pd.DataFrame: Updated DataFrame with path coordinates.\n",
        "    \"\"\"\n",
        "    branch_path_coords = [np.array(skan_skeleton.path_coordinates(i))[:, [2, 1, 0]].tolist() for i in range(len(df_skeleton))]\n",
        "    df_skeleton['path-coords'] = branch_path_coords\n",
        "\n",
        "    return df_skeleton\n",
        "\n",
        "\n",
        "def compute_segments(df_skeleton):\n",
        "    \"\"\"\n",
        "    Computes segment data based on skeleton and path coordinates.\n",
        "\n",
        "    Args:\n",
        "        df_skeleton (pd.DataFrame): DataFrame containing skeleton data.\n",
        "\n",
        "    Returns:\n",
        "        pd.DataFrame: DataFrame containing segments data.\n",
        "    \"\"\"\n",
        "    segments = df_skeleton[['thickness', 'branch-distance', 'euclidean-distance', 'path-coords']].copy()\n",
        "    segments['source_node_id'] = df_skeleton['node-id-src']\n",
        "    segments['destination_node_id'] = df_skeleton['node-id-dst']\n",
        "    segments['tortuosity'] = segments['branch-distance'] / segments['euclidean-distance']\n",
        "    segments.loc[segments['path-coords'].apply(lambda x: x[0] == x[-1]), 'tortuosity'] = -1\n",
        "\n",
        "    return segments\n",
        "\n",
        "\n",
        "def compute_node_coordinates(df_skeleton):\n",
        "    \"\"\"\n",
        "    Computes final node coordinates from skeleton data.\n",
        "\n",
        "    Args:\n",
        "        df_skeleton (pd.DataFrame): DataFrame containing skeleton data.\n",
        "\n",
        "    Returns:\n",
        "        pd.DataFrame: DataFrame containing unique node coordinates.\n",
        "    \"\"\"\n",
        "    final_nodes = pd.concat([\n",
        "        df_skeleton[['node-id-src', 'src-x', 'src-y', 'src-z']].rename(columns={'node-id-src': 'node_id', 'src-x': 'node_coordinate_x', 'src-y': 'node_coordinate_y', 'src-z': 'node_coordinate_z'}),\n",
        "        df_skeleton[['node-id-dst', 'dst-x', 'dst-y', 'dst-z']].rename(columns={'node-id-dst': 'node_id', 'dst-x': 'node_coordinate_x', 'dst-y': 'node_coordinate_y', 'dst-z': 'node_coordinate_z'})\n",
        "    ], ignore_index=True).drop_duplicates().reset_index(drop=True)\n",
        "\n",
        "    return final_nodes"
      ],
      "metadata": {
        "id": "waYHoDmkR9GE"
      },
      "execution_count": 7,
      "outputs": []
    },
    {
      "cell_type": "code",
      "execution_count": null,
      "metadata": {
        "id": "sOxtrTi5E3J7"
      },
      "outputs": [],
      "source": [
        "import os\n",
        "import pandas as pd\n",
        "from zipfile import ZipFile\n",
        "\n",
        "stereom_ids = [1, 2, 3, 4, 5, 6]  # Optionally run on multiple STEREOMs\n",
        "rve_ids = [1, 2, 3, 4]\n",
        "\n",
        "porosity_dict = {}\n",
        "\n",
        "for stereom_id in stereom_ids:\n",
        "    for rve_id in rve_ids:\n",
        "        zip_path = f\"./STEREOM {stereom_id} RVE {rve_id}.zip\"\n",
        "        assert os.path.exists(zip_path), f\"Zip file not found: {zip_path}\"\n",
        "\n",
        "        os.system(f'unzip \"{zip_path}\"')\n",
        "\n",
        "        folder_id_lower = f\"/content/STEREOM {stereom_id} RVE {rve_id}/Threshold/\"\n",
        "        folder_id_upper = f\"/content/STEREOM {stereom_id} RVE {rve_id}/THRESHOLD/\"\n",
        "\n",
        "        folder_id = folder_id_lower if os.path.exists(folder_id_lower) else folder_id_upper\n",
        "\n",
        "        # Unzip the dataset\n",
        "\n",
        "        # raise AssertionError(zip_path)\n",
        "        # with ZipFile(zip_path, 'r') as zip_ref:\n",
        "        #     zip_ref.extractall(\"/content/\")\n",
        "\n",
        "        # os.system(f'unzip \"{zip_path}\"')\n",
        "\n",
        "\n",
        "        # Process TIFF images\n",
        "        segmentation_data_np = tiffs_to_3d_numpy(folder_id)\n",
        "        porosity = compute_porosity(segmentation_data_np)\n",
        "        porosity_dict[f\"STEREOM_{stereom_id}_RVE_{rve_id}\"] = porosity * 100\n",
        "        print(f\"STEREOM {stereom_id}, RVE {rve_id} - Porosity = {porosity * 100:.2f}%\")\n",
        "\n",
        "        # Compute distance transform\n",
        "        dist_trans_3d = compute_distance_transform(segmentation_data_np)\n",
        "\n",
        "        # Skeletonize data\n",
        "        df_skeleton = skeletonize_data(segmentation_data_np, dist_trans_3d)\n",
        "\n",
        "        # Compute branch coordinates\n",
        "        df_skeleton = compute_branch_coordinates(df_skeleton, Skeleton(skeleton_image=skeletonize(segmentation_data_np, method='lee') * dist_trans_3d))\n",
        "\n",
        "        # Compute segments\n",
        "        segments = compute_segments(df_skeleton)\n",
        "\n",
        "        # Save segments to CSV\n",
        "        segments_filename = f\"STEREOM_{stereom_id}_RVE_{rve_id}_segments.csv\"\n",
        "        segments.to_csv(segments_filename, index=False)\n",
        "\n",
        "        # Compute node coordinates\n",
        "        final_nodes_3d = compute_node_coordinates(df_skeleton)\n",
        "\n",
        "        # Save nodes to CSV\n",
        "        nodes_filename = f\"STEREOM_{stereom_id}_RVE_{rve_id}_nodes.csv\"\n",
        "        final_nodes_3d.to_csv(nodes_filename, index=False)\n",
        "\n",
        "        # Plot nodes and branches\n",
        "        plot_3d_nodes_and_branches_mpl(final_nodes_3d, segments)\n",
        "\n",
        "        avg_thickness = df_skeleton['thickness'].mean()\n",
        "        avg_branch_distance = df_skeleton['branch-distance'].mean()\n",
        "        avg_euclidean_distance = df_skeleton['euclidean-distance'].mean()\n",
        "\n",
        "        print(f\"STEREOM {stereom_id}, RVE {rve_id} - Average Thickness: {avg_thickness:.3f}\")\n",
        "        print(f\"STEREOM {stereom_id}, RVE {rve_id} - Average Branch Distance: {avg_branch_distance:.3f}\")\n",
        "        print(f\"STEREOM {stereom_id}, RVE {rve_id} - Average Euclidean Distance: {avg_euclidean_distance:.3f}\")\n",
        "\n",
        "        # Download the generated CSV files\n",
        "        # files.download(segments_filename)\n",
        "        # files.download(nodes_filename)\n",
        "\n",
        "# Save porosity values to a DataFrame\n",
        "porosity_df = pd.DataFrame(list(porosity_dict.items()), columns=[\"Dataset\", \"Porosity (%)\"])\n",
        "print(porosity_df)\n",
        "porosity_df.to_csv(\"porosity_values.csv\", index=False)\n",
        "files.download(\"porosity_values.csv\")\n"
      ]
    },
    {
      "cell_type": "code",
      "source": [
        "import os\n",
        "from zipfile import ZipFile\n",
        "\n",
        "zip_filename = \"/content/all_results.zip\"\n",
        "\n",
        "with ZipFile(zip_filename, 'w') as zipf:\n",
        "    for stereom_id in range(1, 7):\n",
        "        for rve_id in range(1, 5):\n",
        "            nodes_filename = f\"/content/STEREOM_{stereom_id}_RVE_{rve_id}_nodes.csv\"\n",
        "            segments_filename = f\"/content/STEREOM_{stereom_id}_RVE_{rve_id}_segments.csv\"\n",
        "\n",
        "            if os.path.exists(nodes_filename):\n",
        "                zipf.write(nodes_filename, os.path.basename(nodes_filename))\n",
        "            if os.path.exists(segments_filename):\n",
        "                zipf.write(segments_filename, os.path.basename(segments_filename))\n",
        "\n",
        "print(f\"Zipped all nodes and segments CSV files into {zip_filename}\")\n"
      ],
      "metadata": {
        "colab": {
          "base_uri": "https://localhost:8080/"
        },
        "id": "6j6XY22dXcjl",
        "outputId": "067d9477-a340-4934-8478-d21590b4f037"
      },
      "execution_count": 11,
      "outputs": [
        {
          "output_type": "stream",
          "name": "stdout",
          "text": [
            "Zipped all nodes and segments CSV files into /content/all_results.zip\n"
          ]
        }
      ]
    },
    {
      "cell_type": "code",
      "source": [
        "# might take some time\n",
        "# plot_3d_nodes_and_branches_plotly(final_nodes_3d, segments)"
      ],
      "metadata": {
        "colab": {
          "base_uri": "https://localhost:8080/",
          "height": 542
        },
        "collapsed": true,
        "id": "yCTHNu3ASewW",
        "outputId": "ca2bf627-0c4d-4ea8-f42f-880652131184"
      },
      "execution_count": null,
      "outputs": [
        {
          "output_type": "display_data",
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              "                            \n",
              "var gd = document.getElementById('db09d0eb-89e3-4023-9217-6ca98faeeecb');\n",
              "var x = new MutationObserver(function (mutations, observer) {{\n",
              "        var display = window.getComputedStyle(gd).display;\n",
              "        if (!display || display === 'none') {{\n",
              "            console.log([gd, 'removed!']);\n",
              "            Plotly.purge(gd);\n",
              "            observer.disconnect();\n",
              "        }}\n",
              "}});\n",
              "\n",
              "// Listen for the removal of the full notebook cells\n",
              "var notebookContainer = gd.closest('#notebook-container');\n",
              "if (notebookContainer) {{\n",
              "    x.observe(notebookContainer, {childList: true});\n",
              "}}\n",
              "\n",
              "// Listen for the clearing of the current output cell\n",
              "var outputEl = gd.closest('.output');\n",
              "if (outputEl) {{\n",
              "    x.observe(outputEl, {childList: true});\n",
              "}}\n",
              "\n",
              "                        })                };                            </script>        </div>\n",
              "</body>\n",
              "</html>"
            ]
          },
          "metadata": {}
        }
      ]
    },
    {
      "cell_type": "code",
      "execution_count": null,
      "metadata": {
        "id": "2D4H_S2UdqDD",
        "colab": {
          "base_uri": "https://localhost:8080/",
          "height": 18
        },
        "outputId": "9e668420-436a-4e80-eff9-0e6bbe9e7abd"
      },
      "outputs": [
        {
          "output_type": "display_data",
          "data": {
            "text/plain": [
              "<IPython.core.display.Javascript object>"
            ],
            "application/javascript": [
              "\n",
              "    async function download(id, filename, size) {\n",
              "      if (!google.colab.kernel.accessAllowed) {\n",
              "        return;\n",
              "      }\n",
              "      const div = document.createElement('div');\n",
              "      const label = document.createElement('label');\n",
              "      label.textContent = `Downloading \"${filename}\": `;\n",
              "      div.appendChild(label);\n",
              "      const progress = document.createElement('progress');\n",
              "      progress.max = size;\n",
              "      div.appendChild(progress);\n",
              "      document.body.appendChild(div);\n",
              "\n",
              "      const buffers = [];\n",
              "      let downloaded = 0;\n",
              "\n",
              "      const channel = await google.colab.kernel.comms.open(id);\n",
              "      // Send a message to notify the kernel that we're ready.\n",
              "      channel.send({})\n",
              "\n",
              "      for await (const message of channel.messages) {\n",
              "        // Send a message to notify the kernel that we're ready.\n",
              "        channel.send({})\n",
              "        if (message.buffers) {\n",
              "          for (const buffer of message.buffers) {\n",
              "            buffers.push(buffer);\n",
              "            downloaded += buffer.byteLength;\n",
              "            progress.value = downloaded;\n",
              "          }\n",
              "        }\n",
              "      }\n",
              "      const blob = new Blob(buffers, {type: 'application/binary'});\n",
              "      const a = document.createElement('a');\n",
              "      a.href = window.URL.createObjectURL(blob);\n",
              "      a.download = filename;\n",
              "      div.appendChild(a);\n",
              "      a.click();\n",
              "      div.remove();\n",
              "    }\n",
              "  "
            ]
          },
          "metadata": {}
        },
        {
          "output_type": "display_data",
          "data": {
            "text/plain": [
              "<IPython.core.display.Javascript object>"
            ],
            "application/javascript": [
              "download(\"download_8a2cb8af-3af5-432f-8857-7e677dc4a07f\", \"Threshold_nodes.csv\", 38000)"
            ]
          },
          "metadata": {}
        },
        {
          "output_type": "display_data",
          "data": {
            "text/plain": [
              "<IPython.core.display.Javascript object>"
            ],
            "application/javascript": [
              "\n",
              "    async function download(id, filename, size) {\n",
              "      if (!google.colab.kernel.accessAllowed) {\n",
              "        return;\n",
              "      }\n",
              "      const div = document.createElement('div');\n",
              "      const label = document.createElement('label');\n",
              "      label.textContent = `Downloading \"${filename}\": `;\n",
              "      div.appendChild(label);\n",
              "      const progress = document.createElement('progress');\n",
              "      progress.max = size;\n",
              "      div.appendChild(progress);\n",
              "      document.body.appendChild(div);\n",
              "\n",
              "      const buffers = [];\n",
              "      let downloaded = 0;\n",
              "\n",
              "      const channel = await google.colab.kernel.comms.open(id);\n",
              "      // Send a message to notify the kernel that we're ready.\n",
              "      channel.send({})\n",
              "\n",
              "      for await (const message of channel.messages) {\n",
              "        // Send a message to notify the kernel that we're ready.\n",
              "        channel.send({})\n",
              "        if (message.buffers) {\n",
              "          for (const buffer of message.buffers) {\n",
              "            buffers.push(buffer);\n",
              "            downloaded += buffer.byteLength;\n",
              "            progress.value = downloaded;\n",
              "          }\n",
              "        }\n",
              "      }\n",
              "      const blob = new Blob(buffers, {type: 'application/binary'});\n",
              "      const a = document.createElement('a');\n",
              "      a.href = window.URL.createObjectURL(blob);\n",
              "      a.download = filename;\n",
              "      div.appendChild(a);\n",
              "      a.click();\n",
              "      div.remove();\n",
              "    }\n",
              "  "
            ]
          },
          "metadata": {}
        },
        {
          "output_type": "display_data",
          "data": {
            "text/plain": [
              "<IPython.core.display.Javascript object>"
            ],
            "application/javascript": [
              "download(\"download_6fdc6f3c-d3ed-4403-9cb8-1b50260d61db\", \"Threshold_segments.csv\", 668937)"
            ]
          },
          "metadata": {}
        }
      ],
      "source": [
        "# # /content/Thresholds/Threshold model 1/ nodes.csv\n",
        "# files.download(folder_id+\"_nodes.csv\")\n",
        "# files.download(folder_id+\"_segments.csv\")"
      ]
    },
    {
      "cell_type": "code",
      "execution_count": null,
      "metadata": {
        "id": "GdnV_dMmIkqs"
      },
      "outputs": [],
      "source": []
    }
  ],
  "metadata": {
    "colab": {
      "provenance": []
    },
    "kernelspec": {
      "display_name": "Python 3",
      "name": "python3"
    },
    "language_info": {
      "name": "python"
    }
  },
  "nbformat": 4,
  "nbformat_minor": 0
}