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Using PySam for reading and analyzing SAM/BAM files

The Jupyter notebook was created for the purpose of Exercise 2 in the course Genome Informatics. The goal is to read and extract specific information from the BAM file 'merged-tumor.bam'. The data is available as a part of Seven Bridges Genomics public cloud data.

Analysis result

Total number of reads Unmapped reads in the file Reads with with mapping quality 0 Average mapping quality
2,921,629 17,765 126,628 55.91

Requirements

To install necessary libraries, type in shell:

pip install -r requirements.txt