diff --git a/build/Common.props b/build/Common.props
index 8f9490f903..0455be2fb7 100644
--- a/build/Common.props
+++ b/build/Common.props
@@ -34,6 +34,7 @@
       Refer to https://docs.microsoft.com/en-us/nuget/concepts/package-versioning for semver syntax.
     -->
     <MinVerPkgVer>[6.0.0,7.0)</MinVerPkgVer>
+    <MicrosoftAspNetCoreSignalRClientPkgVer>[9.0.0,)</MicrosoftAspNetCoreSignalRClientPkgVer>
     <MicrosoftExtensionsHostingAbstractionsPkgVer>[2.1.0,5.0)</MicrosoftExtensionsHostingAbstractionsPkgVer>
     <MicrosoftExtensionsConfigurationPkgVer>[9.0.0,)</MicrosoftExtensionsConfigurationPkgVer>
     <MicrosoftExtensionsOptionsPkgVer>[9.0.0,)</MicrosoftExtensionsOptionsPkgVer>
diff --git a/src/OpenTelemetry.Instrumentation.AspNetCore/AspNetCoreInstrumentationTracerProviderBuilderExtensions.cs b/src/OpenTelemetry.Instrumentation.AspNetCore/AspNetCoreInstrumentationTracerProviderBuilderExtensions.cs
index 09a60868c6..fa3a322b7f 100644
--- a/src/OpenTelemetry.Instrumentation.AspNetCore/AspNetCoreInstrumentationTracerProviderBuilderExtensions.cs
+++ b/src/OpenTelemetry.Instrumentation.AspNetCore/AspNetCoreInstrumentationTracerProviderBuilderExtensions.cs
@@ -121,5 +121,12 @@ private static void AddAspNetCoreInstrumentationSources(
             builder.AddSource(HttpInListener.ActivitySourceName);
             builder.AddLegacySource(HttpInListener.ActivityOperationName); // for the activities created by AspNetCore
         }
+
+        // SignalR activities first added in .NET 9.0
+        if (Environment.Version.Major >= 9)
+        {
+            // https://github.com/dotnet/aspnetcore/blob/6ae3ea387b20f6497b82897d613e9b8a6e31d69c/src/SignalR/server/Core/src/Internal/SignalRServerActivitySource.cs#L13C35-L13C70
+            builder.AddSource("Microsoft.AspNetCore.SignalR.Server");
+        }
     }
 }
diff --git a/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/BasicTests.cs b/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/BasicTests.cs
index ac7a5929ec..2297a593dd 100644
--- a/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/BasicTests.cs
+++ b/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/BasicTests.cs
@@ -7,6 +7,7 @@
 using Microsoft.AspNetCore.Hosting;
 using Microsoft.AspNetCore.Http;
 using Microsoft.AspNetCore.Mvc.Testing;
+using Microsoft.AspNetCore.SignalR.Client;
 using Microsoft.AspNetCore.TestHost;
 using Microsoft.Extensions.Configuration;
 using Microsoft.Extensions.DependencyInjection;
@@ -1114,6 +1115,63 @@ public async Task ValidateUrlQueryRedaction(string urlQuery, string expectedUrlQ
         Assert.Equal(expectedUrlQuery, activity.GetTagValue(SemanticConventions.AttributeUrlQuery));
     }
 
+#if NET9_0_OR_GREATER
+    [Fact]
+    public async Task SignalRActivitesAreListenedTo()
+    {
+        var exportedItems = new List<Activity>();
+        void ConfigureTestServices(IServiceCollection services)
+        {
+            this.tracerProvider = Sdk.CreateTracerProviderBuilder()
+                .AddAspNetCoreInstrumentation()
+                .AddInMemoryExporter(exportedItems)
+                .Build();
+        }
+
+        // Arrange
+        using (var server = this.factory
+            .WithWebHostBuilder(builder =>
+            {
+                builder.ConfigureTestServices(ConfigureTestServices);
+                builder.ConfigureLogging(loggingBuilder => loggingBuilder.ClearProviders());
+            }))
+        {
+            await using var client = new HubConnectionBuilder()
+                .WithUrl(server.Server.BaseAddress + "testHub", o =>
+                {
+                    o.HttpMessageHandlerFactory = _ => server.Server.CreateHandler();
+                    o.Transports = Microsoft.AspNetCore.Http.Connections.HttpTransportType.LongPolling;
+                }).Build();
+            await client.StartAsync();
+
+            await client.SendAsync("Send", "text");
+
+            await client.StopAsync();
+        }
+
+        WaitForActivityExport(exportedItems, 11);
+
+        var hubActivity = exportedItems
+            .Where(a => a.DisplayName.StartsWith("TestApp.AspNetCore.TestHub", StringComparison.InvariantCulture));
+
+        Assert.Equal(3, hubActivity.Count());
+        Assert.Collection(
+            hubActivity,
+            one =>
+            {
+                Assert.Equal("TestApp.AspNetCore.TestHub/OnConnectedAsync", one.DisplayName);
+            },
+            two =>
+            {
+                Assert.Equal("TestApp.AspNetCore.TestHub/Send", two.DisplayName);
+            },
+            three =>
+            {
+                Assert.Equal("TestApp.AspNetCore.TestHub/OnDisconnectedAsync", three.DisplayName);
+            });
+    }
+#endif
+
     public void Dispose()
     {
         this.tracerProvider?.Dispose();
diff --git a/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/OpenTelemetry.Instrumentation.AspNetCore.Tests.csproj b/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/OpenTelemetry.Instrumentation.AspNetCore.Tests.csproj
index 11def19721..d0bf16a02e 100644
--- a/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/OpenTelemetry.Instrumentation.AspNetCore.Tests.csproj
+++ b/test/OpenTelemetry.Instrumentation.AspNetCore.Tests/OpenTelemetry.Instrumentation.AspNetCore.Tests.csproj
@@ -18,6 +18,7 @@
   <ItemGroup>
     <PackageReference Include="OpenTelemetry.Exporter.InMemory" Version="$(OpenTelemetryCoreLatestVersion)" />
     <PackageReference Include="OpenTelemetry.Extensions.Hosting" Version="$(OpenTelemetryCoreLatestVersion)" />
+    <PackageReference Include="Microsoft.AspNetCore.SignalR.Client" Version="$(MicrosoftAspNetCoreSignalRClientPkgVer)" />
   </ItemGroup>
 
   <ItemGroup>
diff --git a/test/TestApp.AspNetCore/Program.cs b/test/TestApp.AspNetCore/Program.cs
index 5cbe2b5e3a..110693b6fa 100644
--- a/test/TestApp.AspNetCore/Program.cs
+++ b/test/TestApp.AspNetCore/Program.cs
@@ -20,6 +20,8 @@ public static void Main(string[] args)
 
         builder.Services.AddMvc();
 
+        builder.Services.AddSignalR();
+
         builder.Services.AddSingleton<HttpClient>();
 
         builder.Services.AddSingleton(
@@ -43,6 +45,8 @@ public static void Main(string[] args)
 
         app.MapControllers();
 
+        app.MapHub<TestHub>("/testHub");
+
         app.UseMiddleware<CallbackMiddleware>();
 
         app.UseMiddleware<ActivityMiddleware>();
diff --git a/test/TestApp.AspNetCore/TestHub.cs b/test/TestApp.AspNetCore/TestHub.cs
new file mode 100644
index 0000000000..73a8a09bb0
--- /dev/null
+++ b/test/TestApp.AspNetCore/TestHub.cs
@@ -0,0 +1,15 @@
+// Copyright The OpenTelemetry Authors
+// SPDX-License-Identifier: Apache-2.0
+
+using Microsoft.AspNetCore.SignalR;
+
+namespace TestApp.AspNetCore;
+
+public class TestHub : Hub
+{
+    public override Task OnConnectedAsync() => base.OnConnectedAsync();
+
+    public void Send(string message)
+    {
+    }
+}