diff --git a/alembic/script/base.py b/alembic/script/base.py
index d05a54d6..d3cccf6c 100644
--- a/alembic/script/base.py
+++ b/alembic/script/base.py
@@ -211,7 +211,7 @@ def from_config(cls, config: Config) -> ScriptDirectory:
                     )
                 else:
                     version_locations = [
-                        x for x in version_locations_str.split(split_char) if x
+                        x.strip() for x in version_locations_str.split(split_char) if x
                     ]
         else:
             version_locations = None
diff --git a/tests/test_config.py b/tests/test_config.py
index b30f04cc..a5ac0172 100644
--- a/tests/test_config.py
+++ b/tests/test_config.py
@@ -137,7 +137,7 @@ def test_attributes_constructor(self):
         (
             "multiline string 1",
             "newline",
-            "/foo\n/bar",
+            " /foo  \n/bar  ",
             ["/foo", "/bar"],
         ),
         (